P-47: The Effect of Apium graveolens Extract on The Pituitary-Testes Axis in Mice

Background: Celery (Apium graveolens) has many therapeutic effects.This plant has many phytoestrogens that can affect on the reproductive endocrine system and can reduce fertility .In this study, hydro alcoholic extracts of celery leaves on the pituitary-gonad axis in young male l mice of Balb/C was investigated. Materials and Methods: The samples were randomly divided into 5 groups (three treatment groups, placebo and control). Celery leaves hydroalcoholic extract in different doses (50, 100,150 mg/kg/2day) was injected (IP) for 20 days. The Placebo group were used for injection of normale saline. After 10 injections, samples bloods were analyzed for FSH, LH and testosterone by RIA method and compared with control groups. Results: Concentrations of FSH in 100 and 150 mg/kg groups decreased significantly while LH and testosterone concentrations in all experimental groups decreased (p<0.05). Conclusion: This study showed dose-dependent negative effect of celery extracts in the pituitary - gonad axis in male mice.